Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75Article Published on 2022-11-092023-07-10 Journal: Cell Host & Microbe [Category] COVID19(2023년), [키워드] BA.2.75 COVID-19 cryo-EM structure immune evasion neutralizing antibody omicron SARS-CoV-2 spike glycoprotein. [DOI] 10.1016/j.chom.2022.09.018 PMC 바로가기
An antibody that neutralizes SARS-CoV-1 and SARS-CoV-2 by binding to a conserved spike epitope outside the receptor binding motifArticle Published on 2022-10-282022-11-15 Journal: Science Immunology [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] ACE2 receptor acute respiratory syndrome antibody Beta binding conserved coronavirus cryo-EM structure Delta delta variant demonstrated develop DNA epitope Evolution glycosylation site identify immune lung mice neutralize neutralizing activities omicron RBD Receptor binding domain receptor binding motif reduced SARS coronavirus SARS coronaviruses SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2-specific B cell sequenced single-cell the RBD therapeutic variant Viral load viral spike protein were infected [DOI] 10.1126/sciimmunol.abp9962 PMC 바로가기
Virtual Screening and Quantum Chemistry Analysis for SARS-CoV-2 RNA-Dependent RNA Polymerase Using the ChEMBL Database: Reproduction of the Remdesivir-RTP and Favipiravir-RTP Binding Modes Obtained from Cryo-EM Experiments with High Binding AffinityArticle Published on 2022-09-202022-11-15 Journal: International Journal of Molecular Sciences [Category] SARS, 신약개발, 치료법, 치료제, [키워드] acute respiratory syndrome adopted affinity analysis anti-SARS-CoV-2 anti-viral activity approved binding affinity binding free energy ChEMBL Chemistry clinical development Compound coronavirus coronavirus disease COVID-19 Cryo-electron microscopy cryo-EM cryo-EM structure database docking drug drug candidates drug library drug repositioning drugs dsRNA fragment molecular orbital calculation Frequency frequency analysis interactions mode molecular Novel coronavirus ONIOM geometry optimization calculation pathogenic PDB performed predicted quantum quantum chemistry calculation RdRP reported reproduction RNA RNA-dependent RNA polymerase SARS-CoV-2 Screening screenings significantly suggested Treatment Virtual screening virtual screening. was performed [DOI] 10.3390/ijms231911009 PMC 바로가기
Molecular Basis of Mink ACE2 Binding to SARS-CoV-2 and Its Mink-Derived VariantsArticle Published on 2022-09-142022-11-15 Journal: Journal of Virology [Category] SARS, 변종, 유전자 메커니즘, 진단, [키워드] ACE2 acute respiratory syndrome addition angiotensin-converting enzyme 2 Basis binding binding affinity binding mechanism changes in complexes coronavirus Cross-species transmission cryo-EM cryo-EM structure demonstrated examined expressing F486L facilitate highlight hosts Human human cell human monoclonal antibodies humans identify indicated Infection information Interaction mechanism Microscopy mink molecular mechanism Mutation N501T neutralized prevented Protein pseudovirus RBD RBD variant RBD variants receptor reduced residue SARS-CoV-2 SARS-CoV-2 variant sera Structure the binding affinity the RBD the receptor-binding domain the SARS-CoV-2 transmitted variant variants virus entry Virus entry. viruses Y453F [DOI] 10.1128/jvi.00814-22 PMC 바로가기
Flipped over U: structural basis for dsRNA cleavage by the SARS-CoV-2 endoribonuclease뒤집힌 U: SARS-CoV-2 엔도리보뉴클레아제에 의한 dsRNA 절단의 구조적 기초Article Published on 2022-08-122022-09-11 Journal: Nucleic Acids Research [Category] SARS, 유전자 메커니즘, 치료법, [키워드] activate Activation active site cleavage cleave coronavirus coronavirus Coronavirus replication Critical cryo-EM structure cryo-EM structures double-stranded dsRNA endoribonuclease evade form generate Host immune MDA5 mechanism Nsp15 Pattern recognition pattern recognition receptor platform processing recognize replication-transcription complex RNA SARS-CoV-2 SARS-CoV-2 Nsp15 single-stranded site-directed mutagenesis structures substrates the SARS-CoV-2 uridine Vesicle viral replication [DOI] 10.1093/nar/gkac589 PMC 바로가기 [Article Type] Article
Structural basis for inhibition and regulation of a chitin synthase from Candida albicans Research Published on 2022-07-042022-10-05 Journal: Nature Structural & Molecular Biology [Category] 변종, [키워드] binding Candida albican chitin conserved Control cryo-EM structure enzyme activity Extracellular fungal cell wall inhibitor mechanism membrane motif non-specific Prevent Regulation Synthase target translocation unique [DOI] 10.1038/s41594-022-00791-x [Article Type] Research
Quercetin and luteolin are single-digit micromolar inhibitors of the SARS-CoV-2 RNA-dependent RNA polymerase Research Published on 2022-06-222022-10-05 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 신약개발, 치료제, [키워드] activities acute respiratory syndrome ancestor binding mode Compound coronavirus cryo-EM structure docking enzyme Health IC50 inhibit inhibitor Intervention Luteolin pandemic pharmacological quercetin RdRP RdRp inhibitor responsible returned RNA-dependent RNA polymerase SARS-CoV-2 starting point target tested the SARS-CoV-2 these compound These data treat variety viral genome replication viral infections Viral proteins [DOI] 10.1038/s41598-022-14664-2 [Article Type] Research
Structural diversity of the SARS-CoV-2 Omicron spikeSARS-CoV-2 오미크론 스파이크의 구조적 다양성Article Published on 2022-06-022022-09-11 Journal: Molecular Cell [Category] COVID19(2023년), SARS, 변종, [키워드] affecting Analysis changes in communication conformational conformational change Critical cryo Cryo-electron microscopy cryo-EM structure cryo-EM structures Crystallography Delta delta variant determine domain dominant electron microscopy epitope fusion peptide immune immune evasion Impact interprotomer communication intraprotomer communication Mutation Neutralizing Neutralizing antibodies neutralizing antibody omicron Omicron spike peptide RBD Receptor-binding domain SARS-CoV-2 evolution spike Spike protein the SARS-CoV-2 Transmissibility variant vector analysis. virus transmissibility X-ray X-ray crystallography [DOI] 10.1016/j.molcel.2022.03.028 PMC 바로가기 [Article Type] Article
Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617B.1.617에 대한 효능을 유지하는 단일특이성 및 이중특이성 단일클론 SARS-CoV-2 중화 항체Article Published on 2022-03-282022-09-11 Journal: Nature Communications [Category] MERS, SARS, 변종, 유전자 메커니즘, 진단, [키워드] analysis antibody B cell B cells B.1.617 B.1.617.2 binding patterns Breakthrough infection breakthrough infections caused clone complex conformations COVID-19 Critical cryo-EM structure cryo-EM structures death delta variant ectodomain Efficacy Epitopes expand facilitate functional generate humanized identify immunized in vivo inhibitory activity lead mAb mAbs maintain monoclonal monoclonal antibodies monoclonal antibody nanomolar affinity neutralize Neutralizing Neutralizing antibodies neutralizing antibody occur pathogen prefusion Prophylactic RBD Receptor binding domain repertoire SARS-CoV-2 SARS-CoV-2 neutralizing antibody SARS-CoV-2 variant SARS-CoV-2 variants single cell sequencing Spike receptor binding domain spike trimer therapeutic therapeutic option Therapeutics translation Vaccine variants variants of concern virus [DOI] 10.1038/s41467-022-29288-3 PMC 바로가기 [Article Type] Article
Insights into Binding of Single-Stranded Viral RNA Template to the Replication-Transcription Complex of SARS-CoV-2 for the Priming Reaction from Molecular Dynamics Simulations분자 역학 시뮬레이션의 프라이밍 반응을 위한 SARS-CoV-2의 복제-전사 복합체에 대한 단일 가닥 바이러스 RNA 템플릿 결합에 대한 통찰력Article Published on 2022-03-152022-09-11 Journal: Biochemistry [Category] COVID19(2023년), SARS, 치료법, [키워드] bind carried complex cryo-EM structure de novo distribution Dynamics electrostatic electrostatic interactions Evidence include insight molecular molecular dynamics Nsp12 Nsp7 NSP8 nucleotide nucleotides oligomerization primer priming reaction Replication replication-transcription complex responsible RNA RNA polymerase RNA-dependent RNA polymerase RTC SARS-CoV SARS-CoV-2 secondary structure significantly Simulation single-stranded ssRNA stem-loop subunit synthesis tail Template viral genome Viral RNA viral RNAs viral transcription [DOI] 10.1021/acs.biochem.1c00755 PMC 바로가기 [Article Type] Article